Sperm Storage and Transport Following Natural Mating and Artificial Insemination

Fate of fluorescent stained sperm following insemination: new light on oviducal sperm transport and storage in the turkey.

A novel approach was used to evaluate the distribution of sperm in the oviduct of turkey hens inseminated before or after the onset of egg production. Prior to insemination, sperm were stained with the nuclear fluorescent stain bisbenzimide. Sperm distribution in the sperm storage tubules (SST) of the uterovaginal junction and the infundibular tubular glands was determined by use of simultaneou...

Harvesting sperm and artificial insemination of mice.

Rodents of the genus Peromyscus (deer mice) are the most prevalent native North American mammals. Peromyscus species are used in a wide range of research including toxicology, epidemiology, ecology, behavioral, and genetic studies. Here they provide a useful model for demonstrations of artificial insemination. Methods similar to those displayed here have previously been used in several deer mou...

Sperm transport in the female reproductive tract of the brushtail possum, Trichosurus vulpecula, following superovulation and artificial insemination.

This study investigated sperm transport following superovulation and artificial insemination (AI) in the common brushtail possum, Trichosurus vulpecula. Females were superovulated by treatment with 15 IU pregnant mare serum gonadotrophin (PMSG) then 4 mg luteinizing hormone (LH) 78 h later. Inseminations were performed 27 h after LH (4 million motile spermatozoa/uterus). At 1.5, 3, 6, 9 and 12 ...

Development of Ovulation Synchronisation Protocols to Facilitate Natural Mating and Artificial Insemination Breeding Systems

Differences in Mating between a Boar, Traditional Artificial Insemination, and Post Cervical Insemination

As populations around the globe are growing the demand for pork protein in the human diet is increasing. Technologies utilized in 1990s such as artificial insemination have improved our capabilities to produce leaner carcasses. Utilizing superior genetics and breeding techniques as tools to improve growth rates, feed efficiency, and carcass quality is imperative for the swine industry to succes...